Coding

Part:BBa_K4062001:Design

Designed by: Benjamin Bateman   Group: iGEM21_WLC-Milwaukee   (2021-10-15)


lacI ORF


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The 40 base spacer was added because lacY is found downstream of lacZ (~3.1kb) in the e. coli genome. The spacer was added to ensure ample room for regulatory elements or other genes to be added upstream of it.


Source

The ORF and RBS came from the Escherichia coli K-12 sequence on biocyc.org. The spacer contains random bases.

References